BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.

We provide different flow cytometers for your FACS analysis. Becton Dickinson LSRFortessas with up to 17 colours and HTS plate loader. Beckman Coulter

Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3. Check the fluidics. Fludics Default cytometer configuration for the LSR Fortessa analyzer: For more information click the following links: BD LSR Fortessa user manual. BD FACSDiva software reference manual (Version 7.0) BD FACSDiva HTS quick reference guide. HTS quick reference set up guide. BD HTS Instruction manual.

Because many instrument functions are controlled by BD FACSDiva™ software, this guide also contains information document describes how to set up and operate the HTS. JF Fortessa HTS Laser Chart. M Canto Laser Chart JF Fortessa Laser Chart. Protocols. Cell Surface Staining Protocol.

For more information, see the Maintenance chapter in the BD High Throughput Sampler User Guide. Procedure: Placing the HTS in Long-Term Storage. 1 1 3 2 2 3 2 BD FACSAria™ II is discontinued. BD FACSAria™ II, BD FACSAria™ III, BD FACSAria™ Fusion and BD FACSymphony™ S6 are for Research Use Only. Not for use in diagnostic or

provides rapid, fully automated sample acquisition from microtiter BD Fortessa X20 is a state - of - the - art analyser equipped with 5 spatially This cytometer is equipped with HTS - high throughput sampler which allows you to load your samples directly from 96/384 wp. Setup instructions (pictur We provide different flow cytometers for your FACS analysis. Becton Dickinson LSRFortessas with up to 17 colours and HTS plate loader. Beckman Coulter prise en charge de l'option BD HTS sur les appareils BD FACSCanto, nouvelle apparence, possibilité de manuel de référence et du guide de démarrage.

BD High Throughput sampler. The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters

The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design.

BD HTS Instruction manual. Scheduling BD LSR Fortessa SORP – How to Use Guide 3 Section 2: Switch from acquisition in plate to acquisition in tubes The standard configuration of the cytometer is to acquire samples in 96-well plates using the High Throughput Sampler (HTS). If you need to switch the configuration to acquire your samples in FACS tubes, please follow these steps: BD High Throughput sampler.
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For the BD LSR II, the 12 Jan 2019 DETAILED INFO AND PHOTOS FOR THIS CAN BE FOUND ON OUR WEBSITE AT For authorization to use the FACSCanto II cytometer, you first have to receive a training and must accept the user rules of the facility! Tube-based Acquisition. Flow Cthometer Flow cytometer Fortessa Cell Analyser Complete User Guide BD Reference Guide for HTS Option HTS Sampler Daily Action Training BD 7 You are ready to start acquiring your samples using the HTS. Page 4.
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Flow Cytometry Service BD LSR Fortessa X20 – How to Use Guide 5 13 Install the unstained control sample in the cytometer and set the speed to LOW. Start acquisition in the Acquisition Dashboard. 14 In the Cytometer panel, in the Parameters tab, adjust the voltages of FSC and SSC in order to roughly place your cells of interest at the center of the dotplot.

The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. The BD LSRIIs and BD LSR-Fortessa can be configured Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture.

Performance. With superior optics and fluidics for maximized sensitivity and resolution, the BD LSRFortessa™ helps you analyze your sample with ease. It processes 96 samples under 15 minutes with less than 0.5% sample carryover. Cited in over 4,500 publications, the BD …

† A printed copy of the BD High Throughput Sampler User’s Guide is distributed with the BD™ High Throughput Sampler (HTS) option.

2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment. … BD High Throughput sampler. The HTS supports standard 96 (u bottom plates best) and 384 well plates.